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These commensal bacteria colonize the intestinal mucus layer, metabolizing aminoglycans to produce short-chain fatty acids (SCFAs) while stimulating mucin secretion and activating anti-inflammatory pathways (Zhu et al., 2024

Using fragmented mRNA as a template and random oligonucleotides as primers, the first strand of cDNA was synthesized using the M-MuLV reverse transcriptase system, then the RNA chain was degraded by RNaseH, and the second strand of cDNA was synthesized from dNTPs in a DNA polymerase I system

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